Fig. 5From: Dietary L-Methionine modulates the gut microbiota and improves the expression of tight junctions in an in vitro model of the chicken gastrointestinal tractRelative expression of intestinal tight junction genes of methionine supplemented poultry primary intestinal epithelial cells. The gene expression levels related to intestinal tight junction were quantified using qRT-PCR and ∆C(t) values were normalized to internal control GAPDH. All values are expressed as mean ± SD; significant differences were determined using one-way ANOVA followed by Student’s t-test at *P < 0.05, **P < 0.005, ****P < 0.0001 compared to blankBack to article page