Ahmad S, Campos MG, Fratini F, Altaye SZ, Li J. New insights into the biological and pharmaceutical properties of royal jelly. Int J Mol Sci. 2020;21:382.
Article
CAS
PubMed Central
Google Scholar
Pasupuleti VR, Sammugam L, Ramesh N, Gan SH. Honey, propolis, and royal jelly: a comprehensive review of their biological actions and health benefits. Oxid Med Cell Longev. 2017;2017:1259510.
Article
PubMed
PubMed Central
CAS
Google Scholar
Tautz J, Heilmann HR, Sandeman DC, Tautz J. The buzz about bees: biology of a superorganism. Berlin/Heidelberg, Germany: Springer; 2008.
Book
Google Scholar
Li J, Chen S, Su S, Zhong B. Genetic analysis for developmental behavior of honeybee colony’s royal jelly production traits in western honeybees. Acta Genet Sin. 2003;30:547–54.
CAS
PubMed
Google Scholar
Altaye SZ, Meng L, Li J. Molecular insights into the enhanced performance of royal jelly secretion by a stock of honeybee (Apis mellifera ligustica) selected for increasing royal jelly production. Apidologie. 2019;50:436–53.
Article
CAS
Google Scholar
Li J, Feng M, Begna D, Fang Y, Zheng A. Proteome comparison of hypopharyngeal gland development between Italian and royal jelly producing worker honeybees (Apis mellifera L.). J Proteome Res. 2010;9:6578–94.
Article
CAS
Google Scholar
Hu H, Bezabih G, Feng M, Wei Q, Zhang X, Wu F, et al. In-depth proteome of the hypopharyngeal glands of honeybee workers reveals highly activated protein and energy metabolism in priming the secretion of royal jelly. Mol Cell Proteomics MCP. 2019;18:606–21.
Article
CAS
PubMed
Google Scholar
Huo X, Wu B, Feng M, Han B, Fang Y, Hao Y, et al. Proteomic analysis reveals the molecular underpinnings of mandibular gland development and lipid metabolism in two lines of honeybees (Apis mellifera ligustica). J Proteome Res. 2016;15:3342–57.
Article
CAS
PubMed
Google Scholar
Ararso Z, Ma C, Qi Y, Feng M, Han B, Hu H, et al. Proteome comparisons between hemolymph of two honeybee strains (Apis mellifera ligustica) reveal divergent molecular basis in driving hemolymph function and high royal jelly secretion. J Proteome Res. 2018;17:402–19.
Article
CAS
PubMed
Google Scholar
Wu F, Ma C, Han B, Meng L, Hu H, Fang Y, et al. Behavioural, physiological and molecular changes in alloparental caregivers may be responsible for selection response for female reproductive investment in honey bees. Mol Ecol. 2019;28:4212–27.
Article
PubMed
Google Scholar
Han B, Fang Y, Feng M, Hu H, Hao Y, Ma C, et al. Brain membrane proteome and phosphoproteome reveal molecular basis associating with nursing and foraging behaviors of honeybee workers. J Proteome Res. 2017;16:3646–63.
Article
CAS
PubMed
Google Scholar
Zhang X, Hu H, Han B, Wei Q, Meng L, Wu F, et al. The neuroproteomic basis of enhanced perception and processing of brood signals that trigger increased reproductive investment in honeybee (Apis mellifera) workers. Mol Cell Proteomics. 2020;19:1632–48.
Article
CAS
PubMed
PubMed Central
Google Scholar
Kwong WK, Moran NA. Gut microbial communities of social bees. Nat Rev Microbiol. 2016;14:374–84.
Article
CAS
PubMed
PubMed Central
Google Scholar
Moran NA, Hansen AK, Powell JE, Sabree ZL. Distinctive gut microbiota of honey bees assessed using deep sampling from individual worker bees. PLoS ONE. 2012;7:e36393.
Article
CAS
PubMed
PubMed Central
Google Scholar
Kwong WK, Mancenido AL, Moran NA. Immune system stimulation by the native gut microbiota of honey bees. R Soc Open Sci. 2017;4:170003.
Article
PubMed
PubMed Central
CAS
Google Scholar
Zheng H, Powell JE, Steele MI, Dietrich C, Moran NA. Honeybee gut microbiota promotes host weight gain via bacterial metabolism and hormonal signaling. Proc Natl Acad Sci USA. 2017;114:4775–80.
Article
CAS
PubMed
PubMed Central
Google Scholar
Zheng H, Nishida A, Kwong WK, Koch H, Engel P, Steele MI, et al. Metabolism of toxic sugars by strains of the bee gut symbiont Gilliamella apicola. mBio. 2016;7:e01326–e01316.
Article
CAS
PubMed
PubMed Central
Google Scholar
Jones JC, Fruciano C, Marchant J, Hildebrand F, Forslund S, Bork P, et al. The gut microbiome is associated with behavioural task in honey bees. Insectes Soc. 2018;65:419–29.
Article
CAS
PubMed
PubMed Central
Google Scholar
Ludvigsen J, Rangberg A, Avershina E, Sekelja M, Kreibich C, Amdam G, et al. Shifts in the midgut/pyloric microbiota composition within a honey bee apiary throughout a season. Microbes Environ. 2015;30:235–44.
Article
PubMed
PubMed Central
Google Scholar
Hroncova Z, Havlik J, Killer J, Doskocil I, Tyl J, Kamler M, et al. Variation in honey bee gut microbial diversity affected by ontogenetic stage, age and geographic location. PLoS ONE. 2015;10:e0118707.
Article
PubMed
PubMed Central
CAS
Google Scholar
Kešnerová L, Emery O, Troilo M, Liberti J, Erkosar B, Engel P. Gut microbiota structure differs between honeybees in winter and summer. ISME J. 2020;14:801–14.
Article
PubMed
Google Scholar
Liberti J, Engel P. The gut microbiota—brain axis of insects. Curr Opin Insect Sci. 2020;39:6–13.
Article
PubMed
Google Scholar
Powell JE, Martinson VG, Urban-Mead K, Moran NA. Routes of acquisition of the gut microbiota of the honey bee Apis mellifera. Appl Environ Microbiol. 2014;80:7378–87.
Article
PubMed
PubMed Central
CAS
Google Scholar
Martinson VG, Moy J, Moran NA. Establishment of characteristic gut bacteria during development of the honeybee worker. Appl Environ Microbiol. 2012;78:2830–40.
Article
CAS
PubMed
PubMed Central
Google Scholar
Xue Z, Xing’an L, Qinzhi S, Qina C, Chenyi L, Qingsheng N, et al. A curated 16S rRNA reference database for the classification of honeybee and bumblebee gut microbiota. Biodivers Sci. 2019;27:557.
Mandal S, Van Treuren W, White RA, Eggesbø M, Knight R, Peddada SD. Analysis of composition of microbiomes: a novel method for studying microbial composition. Microb Ecol Health Dis. 2015;26:https://doi.org/10.3402/mehd.v26.27663.
Zheng H, Steele MI, Leonard SP, Motta EVS, Moran NA. Honey bees as models for gut microbiota research. Lab Anim. 2018;47:317–25.
Article
Google Scholar
Engel P, Kwong WK, McFrederick Q, Anderson KE, Barribeau SM, Chandler JA, et al. The bee microbiome: impact on bee health and model for evolution and ecology of host-microbe interactions. mBio. 2016;7:e02164-15.
PubMed
PubMed Central
Google Scholar
Kwong WK, Engel P, Koch H, Moran NA. Genomics and host specialization of honey bee and bumble bee gut symbionts. Proc Natl Acad Sci USA. 2014;111:11509–14.
Article
CAS
PubMed
PubMed Central
Google Scholar
Raymann K, Moran NA. The role of the gut microbiome in health and disease of adult honey bee workers. Curr Opin Insect Sci. 2018;26:97–104.
Article
PubMed
PubMed Central
Google Scholar
Emery O, Schmidt K, Engel P. Immune system stimulation by the gut symbiont Frischella perrara in the honey bee (Apis mellifera). Mol Ecol. 2017;26:2576–90.
Article
CAS
PubMed
Google Scholar
Lee FJ, Rusch DB, Stewart FJ, Mattila HR, Newton ILG. Saccharide breakdown and fermentation by the honey bee gut microbiome. Environ Microbiol. 2015;17:796–815.
Article
CAS
PubMed
Google Scholar
Vásquez A, Forsgren E, Fries I, Paxton RJ, Flaberg E, Szekely L, et al. Symbionts as major modulators of insect health: lactic acid bacteria and honeybees. PLoS ONE. 2012;7:e33188.
Article
PubMed
PubMed Central
CAS
Google Scholar
Kwong WK, Medina LA, Koch H, Sing K-W, Soh EJY, Ascher JS, et al. Dynamic microbiome evolution in social bees. Sci Adv. 2017;3:e1600513.
Article
PubMed
PubMed Central
Google Scholar
Bonilla-Rosso G, Engel P. Functional roles and metabolic niches in the honey bee gut microbiota. Curr Opin Microbiol. 2018;43:69–76.
Article
CAS
PubMed
Google Scholar
Kešnerová L, Mars RAT, Ellegaard KM, Troilo M, Sauer U, Engel P. Disentangling metabolic functions of bacteria in the honey bee gut. PLoS Biol. 2017;15:e2003467.
Article
PubMed
PubMed Central
CAS
Google Scholar
Engel P, Martinson VG, Moran NA. Functional diversity within the simple gut microbiota of the honey bee. Proc Natl Acad Sci USA. 2012;109:11002–7.
Article
CAS
PubMed
PubMed Central
Google Scholar
Steele MI, Kwong WK, Whiteley M, Moranb NA. Diversification of type VI secretion system toxins reveals ancient antagonism among bee gut microbes. mBio. 2017;8:e01630-17.
Article
PubMed
PubMed Central
Google Scholar
Kwong WK, Zheng H, Moran NA. Convergent evolution of a modified, acetate-driven TCA cycle in bacteria. Nat Microbiol. 2017;2:1–3.
Article
CAS
Google Scholar
Kwong WK, Mancenido AL, Moran NA. Genome sequences of Lactobacillus sp. strains wkB8 and wkB10, members of the Firm-5 clade, from honey bee guts. Genome Announc. 2014;2:e01176-14.
Zhang W, Zhang X, Su Q, Tang M, Zheng H, Zhou X. Genomic features underlying the evolutionary transitions of Apibacter to honey bee gut symbionts. Insect Sci. 2021.
Raymann K, Bobay L-M, Moran NA. Antibiotics reduce genetic diversity of core species in the honeybee gut microbiome. Mol Ecol. 2018;27:2057–66.
Article
CAS
PubMed
Google Scholar
Motta EVS, Raymann K, Moran NA. Glyphosate perturbs the gut microbiota of honey bees. Proc Natl Acad Sci USA. 2018;115:10305–10.
Article
CAS
PubMed
PubMed Central
Google Scholar
Mysara M, Vandamme P, Props R, Kerckhof F-M, Leys N, Boon N, et al. Reconciliation between operational taxonomic units and species boundaries. FEMS Microbiol Ecol. 2017;93:fix029.
Engel P, Stepanauskas R, Moran NA. Hidden diversity in honey bee gut symbionts detected by single-cell genomics. PLoS Genet. 2014;10:1–13.
Article
CAS
Google Scholar
Ellegaard KM, Engel P. Genomic diversity landscape of the honey bee gut microbiota. Nat Commun. 2019;10:446.
Article
CAS
PubMed
PubMed Central
Google Scholar
Bobay L-M, Wissel EF, Raymann K. Strain structure and dynamics revealed by targeted deep sequencing of the honey bee gut microbiome. mSphere. 2020;5:e00694–20.
Kwong WK, Moran NA. Cultivation and characterization of the gut symbionts of honey bees and bumble bees: description of Snodgrassella alvi gen. nov., sp. nov., a member of the family Neisseriaceae of the Betaproteobacteria, and Gilliamella apicola gen. nov., sp. nov., a member of Orbaceae fam. nov., Orbales ord. nov., a sister taxon to the order “Enterobacteriales” of the Gammaproteobacteria. Int J Syst Evol Microbiol. 2013;63:2008–2018.
Corby-Harris V, Snyder LA, Schwan MR, Maes P, McFrederick QS, Anderson KE. Origin and effect of Alpha 2.2 Acetobacteraceae in honey bee larvae and description of Parasaccharibacter apium gen. nov., sp. nov. Appl Environ Microbiol. 2014;80:7460–7472.
Caporaso JG, Lauber CL, Walters WA, Berg-lyons D, Huntley J, Fierer N, et al. Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. ISME J. 2012;6:1621–4.
Article
CAS
PubMed
PubMed Central
Google Scholar
Magoč T, Salzberg SL. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics. 2011;27:2957–63.
Article
PubMed
PubMed Central
CAS
Google Scholar
Bolyen E, Rideout JR, Dillon MR, Bokulich NA, Abnet CC, Al-Ghalith GA, et al. Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2. Nat Biotechnol. 2019;37:852–7.
Article
CAS
PubMed
PubMed Central
Google Scholar
Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJA, Holmes SP. DADA2: high-resolution sample inference from Illumina amplicon data. Nat Methods. 2016;13:581–3.
Article
CAS
PubMed
PubMed Central
Google Scholar
Price MN, Dehal PS, Arkin AP. FastTree 2—approximately maximum-likelihood trees for large alignments. PLoS ONE. 2010;5:e9490.
Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 2013;30:772–80.
Article
CAS
PubMed
PubMed Central
Google Scholar
Bokulich NA, Kaehler BD, Rideout JR, Dillon M, Bolyen E, Knight R, et al. Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2’s q2-feature-classifier plugin. Microbiome. 2018;6:90.
Article
PubMed
PubMed Central
Google Scholar
Edgar RC. Search and clustering orders of magnitude faster than BLAST. Bioinformatics. 2010;26:2460–1.
Article
CAS
PubMed
Google Scholar
R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. 2020; https://www.R-project.org/.
Bisanz J. qiime2R: Importing QIIME2 artifacts and associated data into R sessions. 2018; https://github.com/jbisanz/qiime2R