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Microbiomes of the Sydney Rock Oyster are acquired through both vertical and horizontal transmission

Abstract

Background

The term holobiont is widely accepted to describe animal hosts and their associated microorganisms. The genomes of all that the holobiont encompasses, are termed the hologenome and it has been proposed as a unit of selection in evolution. To demonstrate that natural selection acts on the hologenome, a significant portion of the associated microbial genomes should be transferred between generations. Using the Sydney Rock Oyster (Saccostrea glomerata) as a model, we tested if the microbes of this broadcast spawning species could be passed down to the next generation by conducting single parent crosses and tracking the microbiome from parent to offspring and throughout early larval stages using 16S rRNA gene amplicon sequencing. From each cross, we sampled adult tissues (mantle, gill, stomach, gonad, eggs or sperm), larvae (D-veliger, umbo, eyed pediveliger, and spat), and the surrounding environment (water and algae feed) for microbial community analysis.

Results

We found that each larval stage has a distinct microbiome that is partially influenced by their parental microbiome, particularly the maternal egg microbiome. We also demonstrate the presence of core microbes that are consistent across all families, persist throughout early life stages (from eggs to spat), and are not detected in the microbiomes of the surrounding environment. In addition to the core microbiomes that span all life cycle stages, there is also evidence of environmentally acquired microbial communities, with earlier larval stages (D-veliger and umbo), more influenced by seawater microbiomes, and later larval stages (eyed pediveliger and spat) dominated by microbial members that are specific to oysters and not detected in the surrounding environment.

Conclusion

Our study characterized the succession of oyster larvae microbiomes from gametes to spat and tracked selected members that persisted across multiple life stages. Overall our findings suggest that both horizontal and vertical transmission routes are possible for the complex microbial communities associated with a broadcast spawning marine invertebrate. We demonstrate that not all members of oyster-associated microbiomes are governed by the same ecological dynamics, which is critical for determining what constitutes a hologenome.

Background

Many host-associated microbiomes play critical roles in host fitness, physiology, immunity, behavior, and development [1]. Animals that are so closely linked to their associated microbes are no longer thought of as individual organisms, instead they are considered holobionts. This term encompasses the multicellular eukaryotic host and all of its associated microorganisms (bacteria, archaea, fungi, protists, and viruses), from obligate symbionts to transient microbes [2,3,4,5]. The genomes of all the members of the holobiont (host and microbiome genomes) can be thought of as the hologenome, which has been proposed as a unit of selection in evolution [2, 5, 6].

There are two main challenges raised against the hologenome concept. The first challenge suggests that if the microbial community and the host do not evolve together, then the hologenome is not real [7,8,9]. The term hologenome however, does not assume coevolution, it simply describes the genomes in the holobiont at a single point in time [10]. Second, questions exist regarding whether or not hologenomes are the primary unit of selection [9]. However, selection can occur at multiple levels of organization including selection on the host genome, the microbiome genomes, and the collective hologenome [2, 6, 10]. The key advance of the hologenome concept is that it promotes awareness that hosts and their associated microbes are tightly linked and that host-associated microbes can affect host fitness [1, 10, 11].

Understanding the evolutionary controls on hosts and their microbiomes across the host’s life cycle is essential to the proper interpretation of the hologenome. Larval development is a key evolutionary process and research is needed to understand how host-associated microbes shift through larval stages, how they shape animal development, and how they influence adult fitness. In vertebrates, the gut microbiome dynamically changes with early developmental milestones [12] and promotes the development of body organs and the immune system [13]. In marine invertebrates, single members of the microbiome can play a critical role in organ development [14]. However, unlike their vertebrate counterparts, it is unclear what role the host-associated microbial community plays in different developmental stages of invertebrates.

In addition to tracking the host microbiomes throughout larval development, it is also essential to disentangle intergenerational transmission modes. If some associated microbes and their genomes play important roles in the evolution of the host, they are likely transmitted between generations [6]. Transmission of associated microbes between generations is usually classified as either vertical, horizontal, or a combination of the two. In horizontal transmission, each new generation of the host acquires associated microbes from the surrounding environment, like the Hawaiian bobtail squid, Euprymna scolopes, and its critical symbiont, Vibrio fischeri [14]. In vertical transmission, associated microbes are passed down to the next generations on or in the gametes, as demonstrated in the marine sponge Chondrilla australiensis [15]. A variation on the definition of vertical transmission of microbes is referred to as maternal transmission, where microbes are transferred from the mother to the offspring, though not necessarily on or in the eggs. Examples of maternal transmission include the inoculation of the offspring through the birthing [16] and breastfeeding [17] processes in mammals.

To build an understanding of the role that microbes play in the health, and ultimately, evolution of organisms, we must determine the mechanism of intergenerational transfer and maintenance of microbial members of the holobiont. While there are examples of vertical transmission of obligate symbionts in some bivalves [18, 19], it is unknown how the complex communities of host microbes are transferred between generations and throughout different life cycle stages. Oysters are an excellent model system to study the role of horizontal and vertical transmission in marine invertebrates. They have been subject to numerous reproduction [20], development [21], and microbiome (reviewed in [22]) studies, which have revealed complex microbial communities in and on oyster larvae [23], and adult tissues [24,25,26]. Additionally, the well-established rearing methods of these commercial species allow for replicated and controlled characterization of host-associated microbes throughout multiple life stages. Oysters are also vital to communities and economies across the globe with their aquaculture production valued at 12 billion USD (United States Dollars) a year [27].

The oyster life cycle begins when sexually mature adults spawn their gametes into the water column, where fertilization and embryonic development take place. After fertilization, the first larval stage, the trochophore, is a free-swimming stage characterized by an apical sensory plate with a tuft of cilia [28]. The next larval stage, the D-veliger, is characterized by the development of the first shell and an organ called the velum that has a ring of cilia for swimming and eating [28]. In the next stage (umbo), larvae develop the protruding shape around the hinge, which gives oysters their distinctive form [28]. The last pelagic larval stage is the eyed pediveliger, characterized by the development of an eye spot and a “foot”, which is used to probe the substrate and find a suitable place to settle [28]. Once it has metamorphosed and settled, the oyster will no longer move and it is referred to as spat until it grows to adult size.

The Sydney Rock Oyster (Saccostrea glomerata) is native to eastern Australia where it forms a 40.9 million USD aquaculture industry [29, 30]. The microbiome of S. glomerata is known to respond to environmental change and possess an associated-bacterial community that is dependent on genotype [31]. Using S. glomerata as a model system, we tested whether oyster-associated microbes are passed down vertically, by conducting single parent crosses and tracking the microbiome from parent to offspring and throughout early larval stages using 16S rRNA gene amplicon sequencing. From each cross, we sampled adult tissues (mantle, gill, stomach, gonad, eggs or sperm), larvae (D-veliger, umbo, eyed pediveliger, and spat), and the surrounding environment (water and algae feed) for microbial community analysis. To determine whether there were horizontally acquired microbes associated with the different developmental stages of oysters, we assessed whether (1) there were microbial communities associated with each developmental stage that were unique to that stage across multiple family lines, and (2) the microbial communities at each larval stage were distinguishable from the surrounding water and food. To assess whether there was vertical transmission of microbes from parent to offspring we determined whether (3) there were microbes that persisted throughout all developmental stages (resulting in the formation of a core microbiome) and if (4) there was a proportion of the microbiome shared between parental gametes and their offspring.

Methods

Sydney Rock Oyster gamete and tissue collection

We purchased 20 adult Sydney Rock Oysters, Saccostrea glomerata, from an oyster farm in Port Stephens, New South Wales (NSW) (-32°45’S, 152°10’E) Australia (Holbert’s Oyster Supplies). These adults formed the parent generation used in our crosses and were approximately 1.5–2 years of age at the time of collection. We transported the oysters to the Department of Primary Industries (DPI) Port Stephens Fisheries Institute (PSFI), Taylors Beach, NSW, Australia, to strip spawn the oysters and make single parent crosses. All seawater used in subsequent experiments and was collected from the estuary adjacent to PSFI (− 32° 44′ 39.2568″ S, 152° 3′ 15.8394″ E) and had a salinity of 34 PSU (practical salinity unit). Seawater was filtered through two 1 μm filter bags and stored in 34,000 L polyurethane tanks onsite.

Once we identified an oyster as a competent (ready to release gametes) female, we removed it from its shell and put it in a sterile petri dish. We scored the gonad tissue with a sterile razor multiple times on both sides and rinsed it with filtered seawater (1 μm filter) to collect the eggs in a sterile glass beaker. Roughly 2000 eggs were pipetted out of the beaker and passed through a stack of sterile, single use cell strainers (pluriStrainer®, pluriSelect Life Science, Leipzig, Germany) to separate debris and capture the eggs. The collection cell strainer (20 μm) with the eggs was rinsed thoroughly with sterile seawater (seawater that was filtered through two 1 μm filter bags and then autoclaved) to remove loosely associated microbes. The cell strainer with the eggs was put in a sterile Whirl–pak (NASCO WHIRL–PAK®, USA) bag, flash-frozen with liquid nitrogen, and stored at − 80 °C until DNA extraction. The remaining eggs in the initial collection beaker were then used for fertilization. We repeated this procedure for each female (n = 5).

When we identified an oyster as a competent male, we removed it from its shell and placed it in a sterile petri dish. We scored the gonad tissue with a sterile razor multiple times on both sides, rinsed it with sterile seawater, and, to remove impurities, filtered the sperm through a single-use, sterile 10 μm pluriStrainer cell strainer and collected the sperm in a sterile falcon tube. Twenty-five μL of sperm were pipetted into a cryovial, flash-frozen in liquid nitrogen, and stored at − 80 °C until DNA extraction. We used the remaining sperm from each male (n = 5) to fertilize the eggs of a specific female, to derive single parent crosses.

Lastly, we collected tissues from the adults used for the crosses. After each adult was rinsed to collect the eggs or sperm, the oysters were transferred back into their shells until the crosses were completed. We then collected mantle, gill, stomach, and gonad tissues for each adult oyster. All of the tissues were thoroughly rinsed with sterile seawater, flash-frozen in liquid nitrogen, and stored at − 80 °C until DNA extraction.

Crosses and larval rearing

We set up five independent single-parent crosses. Fertilization took place in 20 L buckets following the methods of Parker et al. [32]. Buckets were sterilized using Virkon S (Antec International) and filled with 1 μm filtered seawater set at 23 °C. Approximately one million eggs from each female were placed into buckets, sperm was then added incrementally, the mixture was homogenized gently and then a subsample was checked under a light microscope (Leica 200x). This process was repeated until a ratio of 5 sperm per egg was observable in a subsample of the mixture. The gametes were then allowed to rest for 30 min. Fertilized gametes from each cross were then transferred into three 200 L polyethylene larval rearing tanks to create three independent replicates for each single parent cross (n = 15 tanks). Tanks were sterilized using Virkon S and filled with filtered (1 μm) seawater at 23 °C. Larval feeding began after roughly 16 h, with the appearance of the first D-veliger larvae. We fed the larvae twice a day with an algal diet of 50% Chaetoceros muelleri, 25% Diacronema lutheri and 25% Tisochrysis lutea. Algal concentrations started at 1 × 104 cells per mL at the beginning of the experiment and ended at 1.16 × 105 cells per mL at the completion of the experiment as the larvae increased in size. We performed water changes every second day throughout the experiment and followed rearing protocols optimal for S. glomerata larvae that are described in detail in O’Connor et al. [33]. After 33 days the larvae reached the pediveliger stage and showed signs of readiness to settle (eye spot, 300 μm shell length, protruding foot, crawling). We then dosed them with epinephrine to induce settlement, as is commonly done in the hatchery production of this species [33].

Larvae collection

For each single parent cross replicate, we collected multiple stages of larval development: day 1 and day 3 (D-veliger), day 15 (umbo), day 29 (eyed pediveliger), and day 34 (spat). We collected roughly 2,000 individuals from each tank at each larval and spat stage and passed them through a stack of sterile, single-use pluriStrainer cell strainers to separate debris and capture the larvae. The collection cell strainers (40 μm, 70 μm, and 100 μm) with the larvae were rinsed thoroughly with sterile (autoclaved) seawater to remove loosely associated microbes, placed in sterile Whirl–pak bags, flash-frozen with liquid nitrogen, and stored at − 80 °C until DNA extraction. Additionally, we collected seawater and algae feed samples from each tank for microbial community analysis at each of the larval stages. We filtered 500 mL of tank water through a 0.22 μm Sterivex filter. Separately, we pipetted 1 mL of the larvae feed mix into a cryovial. Both water and feed mix were collected in triplicate, flash-frozen, and stored at − 80 °C until DNA extraction.

Nucleic acid preparation and sequencing

We extracted nucleic acids from the adult tissues, gametes, larvae, and environmental samples using the DNeasy PowerLyzer PowerSoil kit (Qiagen, Valencia, CA USA) following the manufacturer’s protocol. To amplify the V4 region of the 16S rRNA gene in triplicate 25 μL polymerase chain reactions (PCR), for all samples we used 5 PRIME Hot Master Mix (Quanta Bio, Beverly, MA USA) and the primers 515FY: 5′TATGGTAATTGTGTGYCAGCMGCCGCGGTAA 3′ [34] and 806RB: 3′ AGTCAGTCAGCCGGACTACNVGGGTWTCTAAT 5′ [35]. The thermocycler conditions were as follows: a 3 min hot start at 94 °C followed by 35 cycles of 94 °C for 45 s, 50 °C for 60 s, and 72 °C for 90 s. The final extension step was 72 °C for 10 min. After checking the triplicate PCR product and negative controls on a gel to ensure the product matched the target size of ~ 390 bp and that there was no contamination, we purified and size selected the PCR products using Agencourt AMPure Magnetic Beads (Beckman Coulter, Brea, CA USA), and resuspended them in 20 μL of nuclease-free water. We then ligated Illumina paired-end adapters with unique Nextera XT v2 indexes to 2 μL of 16S rRNA amplicons using 8 cycles of PCR. The thermocycler conditions were as follows: a 3-min hot start at 95 °C followed by 8 cycles of 95 °C for 30 s, 55 °C for 30 s, 72 °C for 30 s, and a final extension step at 72 °C for 5 min. We then purified and size selected the PCR products using Agencourt AMPure Magnetic Beads, and resuspended them in 20 μL of nuclease-free water. We quantified our libraries using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA USA) and pooled them at equimolar concentrations. After confirming library size on an Agilent 4200 TapeStation (Agilent Technologies, Santa Clara, CA USA), we quantified the library using a KAPA library quantification kit (Roche Sequencing Solutions Inc., Pleasanton, CA USA) and sequenced our library on an Illumina MiSeq with 2 × 250 V2 sequencing chemistry at the Tufts University Core Sequencing Facility.

Procedural controls

We collected procedural negative controls that were carried through library preparation and sequencing. While sampling, we collected negative controls for (1) all cell strainer sizes used to sample gametes and larvae and (2) for the Sterivex filters used to sample seawater microbes. During the library preparation process, we included DNA extraction controls with every batch, and PCR amplification controls. Additionally, we sequenced three replicates of a mock community (ZymoBIOMICS™ Microbial Community DNA Standard, Zymo Research, USA), with known theoretical relative abundances of 10 species, as a positive control. Additional file 1: Fig. S1 illustrates that our mock community replicates were highly consistent with their expected composition.

Sequence analysis

We used the DADA2 (v1.7.0) workflow with default parameters [36], implemented in R Studio (v4.0.0), to quality-filter, merge paired-end reads, remove chimeric sequences, group the sequences into amplicon sequence variants (ASVs), and assign taxonomy against the Silva database (version 132; [37]). Initial processing of the ASV table was conducted using the Phyloseq package [38]. We identified potential procedural and reagent contaminants using the decontam package based on either the frequency of each ASV as a function of the input DNA concentration or the prevalence of each ASV in true samples compared to the prevalence in negative controls [39]. The Decontam package successfully removes 74–91% of contaminants when the source of contamination is not well defined [40]. We assessed the composition of the mock communities to ensure they agreed with the theoretical composition (Additional file 1: Fig. S1). We also filtered out singleton ASVs and ASVs identified as mitochondria, chloroplasts, Eukaryota, and Archaea, which accounted for less than 3% of our data set. Samples with less than 1000 reads after quality filtering were removed from the data set (n = 13 of 192). Rarefaction analyses confirmed that the sequencing coverage was sufficient to get the vast majority of the bacterial diversity in all oyster tissue samples (Additional file 1: Fig. S2). To account for uneven sequencing depths across samples, we (1) transformed our data to proportions by dividing the reads for each ASV in a sample by the total number of reads in that sample, as previously recommended [41,42,43,44,45] to conduct β diversity analyses, and (2) normalized reads by converting ASV abundances to Z-scores before running Random Forrest classification models. The rest of our statistical analyses relied on presence/absence data of samples that were sequenced deeply enough to have representative diversity.

Statistical analysis

To test our first two hypotheses of whether (1) different larval stages harbor distinct microbial communities and (2) larvae microbiomes differ from water and algae microbiomes, we focused on β diversity and computed a Sorensen-Dice dissimilarity matrix using the vegdist function in Vegan [46]. We used this dissimilarity matrix to run three independent permutational multivariate analysis of variance (PERMANOVA), with larval stage, environment, and family as factors all with 999 permutations using the adonis2 function in Vegan [46]. We ran post-hoc pairwise comparisons, with Bonferroni corrected P-values, to compare larval stages to each other, to the gametes, and to their surrounding environment, using the custom function pairwise.adonis (https://github.com/pmartinezarbizu/pairwiseAdonis; [47]). We also tested for homogeneity of multivariate dispersions using betadisper, and ran post-hoc pairwise comparisons using the permutest.betadisper function in Vegan [46]. To visualize β diversity of the different larval stages, gametes, and their surrounding environment, we used non-metric multidimensional scaling (NMDS) plots of the Sorensen-Dice dissimilarities using the metaNMDS package in Vegan [46]. We carried out our statistical analyses following the recommendations of the Guide to Statistical Analysis in Microbial Ecology [48].

To further investigate the microbial communities associated with different larval stages, we performed a network analysis using Gephi [49] to determine the distribution of ASVs (relative abundance > 0.001). Gephi uses a force-directed graph algorithm to visualize the network (ForceAtlas2). We then conducted modularity analysis on the network (Louvain), and ran a Random Forest Classification model using 10,001 trees on a feature table containing ASVs that were in more than 20% of the samples (179 ASVs total) to find the ASVs that best describe each larval stage (randomForest R package; [50]). Model performance was confirmed by examining the out-of-bag error rate and we performed leave-one-out cross-validation with 999 permutations in the caret R package [51].

To test our second two hypotheses regarding (3) whether there was a portion of the microbiome (a core) that persisted across all life cycle stages and (4) whether larvae shared a portion of their microbiome with their parent gametes, we focused on oyster-specific ASVs. We used set theory functions in R and a more conservative relative abundance cut off of 0.01 to find ASVs present in oyster samples that were not detected in the oyster environment (tank water and algae). These oyster-specific ASVs were then used to investigate whether any microbes persisted across all life cycle stages. To do this we performed a core analysis with a relative abundance > 0.01 and prevalence > 0.5, as defined in previous studies [52], using the core_members function in the microbiome R package [53]. We also used the oyster-specific ASVs to investigate whether there was a portion of the larvae microbiome shared with parent gametes. We used set theory functions in R and performed pairwise comparisons of all parent and offspring pairs to find the percentage of ASVs in the offspring that were shared with their parent eggs and sperm. To accompany this, we performed a Mann–Whitney U test to compare whether offspring shared more ASVs with their parent eggs or sperm. All figures were created using ggplot2 [54].

Results

We found that each larval stage harbored compositionally distinct microbiomes that were different from their surrounding environment (Fig. 1). Non-metric multidimensional scaling (NMDS) ordination plots of the Sorensen-Dice dissimilarity indices showed distinct larvae-associated microbial communities that clustered by adult tissue types, larval stage, and gametes (Fig. 2a), and that were also distinct from the tank water and algae (Fig. 2b). Furthermore, permutational multivariate analysis of variance (PERMANOVA) models showed a significant (P < 0.001) effect of larval stage on microbial community composition (Additional file 1: Table S1), with pairwise significant differences (P < 0.05) between all pairs of larval stages except between day 29 (eyed pediveliger) larvae and day 34 spat (P > 0.05; Additional file 1: Table S1). Generally, adult tissues were different from larval stages, gametes, and each other (P < 0.05). PERMANOVA models also showed that larval microbiomes were distinct from tank water and algae feed (P < 0.001) at all larval stages (Additional file 1: Table S2). When considering all larval stages, individual families did not have distinct microbiomes (Fig. 2c).

Fig. 1
figure 1

Stacked bar plot of the relative abundance of bacterial orders comprising microbial communities associated with oyster tissues (gill, mantle, stomach, gonad, gametes and larvae) and their environment (water and algae). Relative abundance was calculated within each sample and ASVs that made up less than 1% of the sample were excluded

Fig. 2
figure 2

Non-metric multidimensional scaling (NMDS) plots of Sorensen-Dice dissimilarities of microbial communities associated with oyster larvae, gametes, and their environment. Colored by A larval stage day, adult tissues and gametes, B larvae stage day and environment, and C family. Group centroids are defined by the mean dissimilarities for each group

We found high inter-individual variability throughout all life stages, however, this variability was especially noticeable in sperm, eggs, and older larvae (Fig. 1). We observed changes over time in the larvae and water microbiomes, with the bacterial order Alteromonadales having greater relative abundance in younger larvae and decreasing in abundance over time (Fig. 1). Many of the Alteromonadales ASVs that were highly abundant at earlier larval stages were also abundant in water samples (Additional file 1: Fig. S3), although their relative abundance patterns in early larval stages do not resemble the relative abundance patterns in the water samples. There were also Alteromonadales ASVs that were unique to oyster tissues and not detected in the water or algae samples (Additional file 1: Fig. S3), possibly these ASVs were present in such low abundances in water or algae samples that we did not detect them using our sampling methods. Other bacterial orders like Cellvibrionales increased in abundance through the umbo stage and remained abundant in some families but not others, through the spat stage (Fig. 1).

We observed compositional differences in the microbial community depending on larval stages, gamete type, and the environment. However, when examining specific ASVs, we found many ASVs that were shared among multiple larval stages. Network analysis allowed us to visualize the distribution of ASVs among larvae, adults, seawater, and algae samples. ASVs unique to a single group accounted for the largest fraction of ASVs (3724 of 4420), while ASVs shared by all groups were the smallest fraction of the total (127 of 4420). Modularity analysis indicates regions of the network that are more closely connected and in this network we identified 8 such modules (Fig. 3). The modularity analysis revealed that earlier larval stages, D-veligers (day 1) and D-veligers (day 3) form one module indicating that they have many ASVs in common, similarly, later larval stages day 29 (eyed pediveligers) and day 34 (spat) were also grouped in one module. All other sample types (adults, eggs, sperm, umbo, water, and algae) were identified as unique modules indicating their distinctness (Fig. 3).

Fig. 3
figure 3

Force directed network of the ASVs in our data set with a relative abundance > 0.001 (n = 4420). Every dot identifies an ASV and the edges connect the ASVs to the sample type they were found in. Colors represent different modules identified in modularity analysis of the network

The random forest model that determined which ASVs best describe each larval stage, correctly classified the larvae microbial communities as belonging to the different larval stages 75.91% of the time with a 26.09% out-of-bag error rate. Older larval stages were more difficult for the model to predict than younger larval stages, 100% of day 1 (D-veligers), 90% of day 3 (D-veligers), and 88% of day 15 (umbo) larvae were classified correctly, while 0% day 29 (eyed pediveligers), and 33% of day 34 (spat) were classified correctly. Leave-one-out cross-validation confirmed model performance, with a Cohen’s kappa statistic of 71.72%. Of the top 30 ASVs that best describe each larval stage, 18 of them were Gammaproteobacteria, 10 were Alphaproteobacteria, one was a Deltaproteobacteria, and one was a Bacteroidia (Fig. 4).

Fig. 4
figure 4

Heat map of the 30 ASVs that are the most important contributors to a Random Forest classification model that was trained to predict larval stage from microbial community composition. The heat map shows the relative abundance of the ASVs in samples of the different larval stages (Day), samples are clustered using Sorensen-Dice dissimilarity distances. More detailed taxonomic information can be found in Additional file 1: Table S4. Stars denote ASVs that were not detected in tank water and algae samples with a relative abundance greater than 1%

We found 385 ASVs that were unique to oyster adult and larvae tissues and were not detected in the environment (tank water and algae). A core analysis of these oyster-specific microbes, indicated ASVs that were maintained across many, if not all, life cycle stages within each family. We found a total of 14 core ASVs including, ASV_33 and ASV_11 belonging to the Orders Rhodobacterales and Vibrionales respectively, in the eggs and all larval stages with increasing relative abundance through time (Fig. 5). ASV_44 in the Order Alteromonadales, was observed in the eggs and sperm of nearly all families and persisted in the larval stages of nearly all families. Additionally, we found that offspring shared significantly more ASVs with their parent eggs than with their parent sperm (Mann–Whitney, day 1: P = 0.05, day 3: P = 0.04, day 15: P = 0.02, day 29: P = 0.002, day 34: P > 0.05; Fig. 6A; Additional file 1: Table S6), and that a portion of the microbiome was conserved between larval stages as they grew older (Fig. 6B).

Fig. 5
figure 5

Box and whisker plots of the relative abundances of ASVs identified as core members (present at greater than 1% abundance in more than 50% of samples) in the eggs, sperm, and all larval stages. This analysis excluded all ASVs detected in tank water and algae samples with a relative abundance greater than 1%. More detailed taxonomic information can be found in Additional file 1: Table S5

Fig. 6
figure 6

Box and whiskers plots of the percentage of ASVs shared A between each larval stage and their parent eggs and sperm, and B between adjacent larval stages. This analysis excluded all ASVs detected in tank water and algae samples with a relative abundance greater than 1%

Discussion

Elucidating how microbes are transferred across generations and maintained across the larval cycle is critical to our understanding of the role of microbes in the evolution of animals. We tracked the presence of microbes across parent gametes, larval stages, and their surrounding environment (i.e., tank water and algae feed) and identified microbes that are potentially transferred vertically and, based on existing literature, may infer benefits to the host. We found that early oyster larvae shared a portion of their microbiome with parent eggs and sperm suggesting the possible transmission of microbes from their parents, primarily via eggs. Subsequent development of the larval microbiome was then consistently parallel across families, suggesting horizontal transmission of larval stage-specific microbes from their environment. These findings support the holobiont concept by demonstrating that microbes were consistently associated with gametes and specific larval stages across five independent families. We showed that (1) different developmental stages had distinct microbiomes, and (2) the microbial communities at each larval stage were distinguishable from the surrounding water and food. We also assessed whether there was vertical transmission of microbes from parent to offspring by determining that (3) there were microbes that persist throughout all developmental stages and that (4) there was a proportion of the microbiome shared between offspring and their parental gametes, particularly the eggs. Thus, our findings provide evidence for both horizontal and vertical transmission strategies being used to transfer the complex communities of microbes across larval stages of a broadcast spawning marine species. We also highlight key taxa that are conserved across parent, offspring, and developmental stages suggesting that they potentially play important roles in oyster fitness.

Larvae microbiomes vary with host developmental stages

Animal development is influenced by and, in some cases, depends on their associated microbiome. Like many marine invertebrates, oyster life history involves distinct planktonic and sessile phases. Our results support the hypothesis that oyster larvae-associated microbial communities change across developmental stages, with taxonomic membership shifting in consistent ways across multiple family lines throughout life history stages from eggs to spat. These consistent shifts suggest specific life-cycle stage selection of microbes from the environment via horizontal transmission. This is consistent with patterns observed in bacterial communities of fish larvae [55, 56], shrimp larvae [57] and Eastern oyster larvae [58]. The specificity of microbial communities at each larval stage suggests that the host environment shapes a portion of the microbial community in planktonic marine larvae through horizontal acquisition of microbes.

When we clustered our larvae microbiome samples based on shared taxa (Fig. 4); earlier larval stages generally clustered together and were separate from the late larval stages. This partitioning between earlier and later larval stages indicates a gradual progression in the microbial community composition between adjacent stages. This pattern has also been observed in sea urchin larvae, where their bacterial communities clustered based on shared taxa by sequential developmental stages [59]. It was surprising that we did not observe more abrupt shifts between major developmental milestones such as when larvae begin feeding or metamorphose into spat. This may be because during metamorphosis oyster larvae reabsorb larval organs, such as the foot and velum. The spat may have retained, instead of shed [60], a portion of the eyed pediveliger microbial community. There may also be a lag between metamorphosis and the time it takes for a new spat-specific microbial community to assemble.

Some of the ASVs identified as important contributors to the microbiomes of day 1 and day 3 larvae (D-veliger; ASV_4, ASV_31, ASV_130) belong to the class Gammaproteobacteria, family Alteromonadaceae (Fig. 4). ASV_44, a member of the core microbiome (Fig. 5) was also a member of the Alteromondaceae and was prevalent in the eggs, and particularly in the sperm, as well as in several subsequent larval stages, suggesting it may be a key component of vertical transmission. Members of this family have also been identified as dominant in D-veliger larvae of Pacific oysters [61]. Potential beneficial roles of Alteromonadaceae include protection against pathogens [62] and provision of fixed nitrogen to their animal host [63]. The protection and nutritional properties of this bacterial family could confer benefits to the host during the early more vulnerable life stages of oyster larvae. Additionally, members of this family were detected in oyster tissues but not in their environment (Additional file 1: Fig. S3). Possibly these ASVs were present in tank water and algae in such low abundances that we were unable to detect them using our sampling methods. Nonetheless, considering their notable abundance in the D-Veliger stage and their prevalence in other oyster microbiomes suggests more effort should be directed at understanding their role in oyster larval development. Some ASVs that were important at later larval stages, day 15 (umbo), day 29 (eyed pediveliger) and day 34 (spat; ASV_26, ASV_33, ASV_125), were members of the class Alphaproteobacteria, family Rhodobacteraceae (Fig. 4). Bacteria of this family are widespread in marine ecosystems and are commonly associated with marine eukaryotes [64]. Many host-associated Rhodobacteraceae strains produce vitamin B12 [64, 65], which can radically alter gene expression and can accelerate development and reduce mortality in invertebrate hosts [66, 67]. Members of this family were also identified in high abundance in pre-settlement Pacific oyster larvae [61], and they may play a key role in the B12 supply of late larval stages. Additionally, host-associated Rhodobacteraceae strains produce an assortment of extracellular signaling compounds [65] that may be involved in controlling the physiological activities of host-associated bacterial communities. The nutritional and signaling properties of this family may be important to pre-settlement larval stages as they prepare to metamorphose.

Larvae microbiomes are different from their environment

In addition to demonstrating a distinct and consistent progression during larval stages, our findings also indicate that larvae microbiomes were not merely a reflection of their surrounding water and food. These data are consistent with previous studies of marine invertebrate larvae in wild [68] and hatchery [23, 57, 58, 69] settings. Despite maintaining a distinct microbiome, the environment plays an important role in the initial establishment of host-associated microbes [13, 16]. We found that seawater microbes seem to be more important in influencing the microbiomes of earlier larval stages (D-veligers) than later larval stages (eyed pediveligers and spat) (Fig. 1; Additional file 1: Fig. S3). Similarly, shrimp larval microbes were influenced at earlier but not later larval stages by the rearing water microbiomes [57]. Our findings suggest that microbes in the environment may be important to the establishment of the microbiomes of earlier oyster larvae stages. This is of relevance to the oyster aquaculture industry and oyster conservation efforts because the D-veliger stage may be the most vulnerable to environmental microorganisms.

The influence of diet on marine larvae microbiomes is inconsistent in the literature. Some studies found a strong influence of diet-associated microbes on larval stages [70], while others found no effect of food microbes on larvae microbiomes [55]. Our findings agree with the latter, as we found that algae feed did not contribute significantly to oyster larvae microbiomes. It is possible that algae feed did not contribute to shifts in the larvae because we maintained the same algal diet throughout the experiment. By contrast, studies that change the feed source depending on the larval stage had larvae microbiome changes that coincided with changes in diet [70]. Further work is needed to determine the contribution of diet to the formation of aquatic larvae microbiomes. Experiments that focus on a single larval stage and have different food types as a factor would be particularly illustrative. Divergence from algal and tank water microbial communities suggests that oyster larvae select at least part of their associated microbial communities, rather than mirror the communities present in their surroundings, as seen in other invertebrates [71]. The consistent patterns of microbes selected at each larval stage across all oyster family lines suggest they are acquired via horizontal transmission.

Some microbes persist throughout all developmental stages

We found that there was a core microbiome, not detected in the environment, that was persistent across multiple life cycle stages, including gametes, which suggests this may be vertically transmitted. The ASVs that were consistently found throughout the larval cycle and in different families include microbes with known symbiotic features and functional capabilities. Two of the ASVs identified in the core microbiome started in low relative abundances in the eggs and slowly increased in relative abundance through larval stages, resulting in a higher abundance in the spat stage relative to the eggs (Fig. 5). One of the ASVs that followed this pattern was ASV_33, belonging to the order Rhodobacterales, family Rhodobacteraceae, genus Nautella. This genus of bacteria is thought to be deposited into the egg cases of cephalopod mollusks where they are hypothesized to play a role in egg defense [72, 73]. Host-associated members of this family also produce vitamin B12 [64, 65] which can play a role in invertebrate development [66, 67]. This ASV was identified by both our core analysis (Fig. 5) and the Random Forest model that determined which ASVs best describe each larval stage (Fig. 4), the maintenance and abundance patterns of this ASV suggest that its functional importance should be investigated due to its prevalence on Sydney Rock Oyster larvae.

Another core ASV with a unique relative abundance pattern was ASV_44. This ASV had the highest mean relative abundance in sperm samples relative to its abundance in eggs and larval stages, and it belongs to the order Alteromonadales, genus Pseudoalteromonas. Strains from this genus were previously isolated from oyster hemolymph and shown to have antibacterial activity against gram-negative bacteria including Vibrio strains that are pathogenic to oysters [74]. It is possible that Pseudoalteromonas could protect sperm from detrimental bacteria that cause sperm agglutination and reduce sperm success [75]. This biologically active genus may also help create an extracellular environment in the seminal plasma suitable for sperm activation success [76, 77]. Although previous studies have found relationships between microbial community structure and sperm fertilization success [78, 79] further work is necessary to determine the function of sperm-associated bacteria.

Some ASVs identified in the core analysis had patchy relative abundance patterns across life history stages, such that they were abundant at some larval stages but not at others, possibly indicating their importance at a specific larval stage. For example ASV_141, ASV_121, and ASV_68, which had patchy abundance patterns, belong to the order Oceanospirillales (Fig. 5). Members of this order are commonly found in association with oyster larvae [23] and adults [22], and are symbiotic with the gills of many bivalves [80,81,82]. Additionally, they are known for their capacity to break down organic compounds in the environment and their abundance in crude-oil containing seawater [83, 84]. Their symbiotic capabilities with bivalves indicate that Oceanospirillales may confer beneficial effects to their larvae host and may be more useful at some larval stages than others. Within this order, ASV_121, family Saccharospirillaceae, genus Bermanella, was particularly interesting because of its high abundance in the eggs and first larval stage. Members of this genus produce poly-β-hydroxybutyrate (PHB) [85]. PHB is an energy storage compound produced by multiple types of bacteria [85,86,87] and has been demonstrated to increase survival and growth in various marine organisms [88,89,90] and protect marine larvae from infection by Vibrio pathogens [91, 92]. It is possible that Bermanella could have a beneficial association with oyster eggs and larvae by inhibiting pathogens and promoting growth.

Larvae share a portion of their microbiomes with parent gametes and adjacent larval stages

We found that oyster larvae shared a higher percentage of ASVs with their parent eggs than sperm, suggesting that maternal contributions to larvae were more important than paternal contributions. This finding is not surprising considering that maternal provisions of microbes to offspring via the eggs is widespread in marine and terrestrial animals, with examples spanning multiple phyla ranging from Porifera, Molluska, Arthropoda, and Chordata (reviewed in [93]). Larvae shared a relatively small percentage of their microbes with their parent sperm, which suggest a small paternal contribution. This finding is also consistent with other examples of sperm-mediated vertical transmission in the marine environment [15]. Although there are multiple examples of biparental transmission of microbes in invertebrates [15, 94, 95] our study documents biparental modes of vertical transmission in oysters.

We also found that adjacent larval stages shared a relatively high percentage of their microbiomes (Fig. 6B). This suggests that, despite family line, larvae acquired the same microbes from the environment and conserved a portion of them throughout the gradual succession of microbiomes during development. Gradual succession patterns in other marine invertebrate larvae have been previously documented [57, 59]. These findings suggest that from early to late stages of larval development some members of their microbiome persist, some are lost, and some are gained along the way. The microorganisms that persist alongside the ones that are gained and carried forward are likely important to host fitness.

Conclusions

The hologenome theory of evolution relies on the assumption that parents consistently transfer beneficial microbes to their offspring. We tested this assumption by using Sydney Rock Oysters as a model and conducting single parent crosses to track the microbiomes from parents to offspring. We characterized the succession of oyster larvae microbiomes from gametes to spat. We found that oyster-associated bacterial communities varied with host developmental stage, differed from their environment microbiomes, and had select members that persisted across multiple life stages. We also demonstrate that both vertical and horizontal transmission routes are possible for the complex communities of microbes associated with broadcast spawning marine invertebrates and that not all members of the oyster microbiome are governed by the same ecological dynamics. The functional importance, evolutionary significance, and mechanisms that drive changes throughout life history stages of oyster-associated microbial communities require additional investigation. Future experiments involving combined molecular microbiological (e.g. metagenomics) and geochemical approaches that can link microbial identity with metabolic activity are needed to explicitly test the functional relevance of host-associated microbiomes that are both vertically and horizontally transmitted across generations.

While our experiment does not directly test the hologenome theory of animal microbiomes, our results contribute to the ongoing conversation of this heavily debated topic (reviewed in [2, 10]). Some of our results are relevant to the assumptions posed by the hologenome theory of evolution. To determine if the hologenome is a level of selection, one of the critical assumptions is of consistent microbiome transmission from one holobiont generation to the next [2]. Theoretically, ‘consistent microbiome transmission’ is defined as offspring microbiota being more similar to the microbiomes of their parents than to those of other unrelated adults in the population [2]. Our findings, however, suggest that vertically and horizontally transmitted microbes were not different depending on oyster family, but they were rather consistently replicated across oyster families. This means that different oyster families were transferring the same microbes to their offspring. Our finding is consistent with a previous study that showed that vertically transmitted microbes were not faithful to a single marine sponge species [96]. While our results suggest that intergenerational transfer of oyster microbes is possible, they do not necessarily fit the current definition of the ‘consistent microbiome transmission’ assumption of the hologenome theory. Further testing is required to determine whether the patterns reported here persist across other marine broadcast spawning marine organisms.

Availability of data and materials

The datasets generated and analyzed during the current study are available in the NCBI SRA database under BioProject ID no. PRJNA 761,332. https://www.ncbi.nlm.nih.gov/bioproject/761332.

Abbreviations

USD:

United States Dollars

NSW:

New South Wales

DPI:

Department of Primary Industries

PSFI:

Port Stephens Fisheries Institute

PSU:

Practical salinity unit

PCR:

Polymerase chain reaction

ASV:

Amplicon sequence variant

NMDS:

Non-metric multidimensional scaling

PERMANOVA:

Permutational multivariate analysis of variance

References

  1. McFall-Ngai M, Hadfield MG, Bosch TCG, Carey HV, Domazet-Lošo T, Douglas AE, et al. Animals in a bacterial world, a new imperative for the life sciences. Proc Natl Acad Sci. 2013;110:3229–36.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Bordenstein SR, Theis KR. Host biology in light of the microbiome: ten principles of holobionts and hologenomes. PLoS Biol. 2015;13:1–23.

    Article  CAS  Google Scholar 

  3. Gordon J, Knowlton N, Relman DA, Rohwer F, Youle M. Superorganisms and holobionts. Microbe Mag. 2013;8:152–3.

    Article  Google Scholar 

  4. Rohwer F, Seguritan V, Azam F, Knowlton N. Diversity and distribution of coral-associated bacteria. Mar Ecol Prog Ser. 2002;243:1–10.

    Article  Google Scholar 

  5. Rosenberg E, Koren O, Reshef L, Efrony R, Zilber-Rosenberg I. The role of microorganisms in coral health, disease and evolution. Nat Rev Microbiol. 2007;5:355–62.

    Article  CAS  PubMed  Google Scholar 

  6. Rosenberg E, Zilber-Rosenberg I. Adaptation, development, and evolution. Role Microorg Adapt Dev Evol Anim Plants Hologenome Concept. 2013;346–58.

  7. Chandler JA, Turelli M. Comment on “The hologenomic basis of speciation: Gut bacteria cause hybrid in the genus Nasonia.” Science. 2014;345:1011.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Hester ER, Barott KL, Nulton J, Vermeij MJA, Rohwer FL. Stable and sporadic symbiotic communities of coral and algal holobionts. ISME J. 2015;10:1157–69.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  9. Moran NA, Sloan DB. The hologenome concept: helpful or hollow ? PLoS Biol. 2015;13: e1002311.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  10. Theis KR, Dheilly NM, Klassen JL, Gilbert SF, Goodnight CJ, Lloyd EA, et al. Getting the hologenome concept right: an eco-evolutionary framework for hosts and their microbiomes. Am Soc Microbiol. 2016;1:e00028-e116.

    Google Scholar 

  11. Bosch TCG, McFall-Ngai MJ. Metaorganisms as the new frontier. Zoology. 2011;114:185–90.

    Article  PubMed  Google Scholar 

  12. Koenig JE, Spor A, Scalfone N, Fricker AD, Stombaugh J, Knight R. Succession of microbial consortia in the developing infant gut microbiome. PNAS. 2010;108:4578–85.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Sommer F, Bäckhed F. The gut microbiota-masters of host development and physiology. Nat Rev Microbiol. 2013;11:227–38.

    Article  CAS  PubMed  Google Scholar 

  14. Nyholm SV, McFall-Ngai MJ. The winnowing: establishing the squid—vibrios symbiosis. Nat Rev Microbiol. 2004;2:632–42.

    Article  CAS  PubMed  Google Scholar 

  15. Usher KM, Sutton DC, Toze S, Kuo J, Fromont J. Inter-generational transmission of microbial symbionts in the marine sponge Chondrilla australiensis (Demospongiae). Mar Freshw Res. 2005;56:125–31.

    Article  Google Scholar 

  16. Dominguez-bello MG, Costello EK, Contreras M, Magris M, Hidalgo G. Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns. PNAS. 2010;107:11971–5.

    Article  PubMed  PubMed Central  Google Scholar 

  17. Martín V, Maldonado-barragán A, Moles L, Rodriguez-baños M, Campo R, Rodríguez JM, et al. Sharing of bacterial strains between breast milk and incant feces. J Hum Lact. 2012;28:36–44.

    Article  PubMed  Google Scholar 

  18. Endow K, Ohta S. Occurrence of bacteria in the primary oocytes of vesicomyid clam Calyptogena soyoae. Mar Ecol Prog Ser. 1990;64:309–11.

    Article  Google Scholar 

  19. Cary SC, Giovannoni SJ. Transovarial inheritance of endosymbiotic bacteria in clams inhabiting deep-sea hydrothermal vents and cold seeps. Proc Natl Acad Sci. 1993;90:5695–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Marshall DA, Moore SC, Sutor M, La Peyre JF, La Peyre MK. Using reproductive potential to assess oyster population sustainability. Restor Ecol. 2020;28:1621–32.

    Article  Google Scholar 

  21. Vogeler S, Bean TP, Lyons BP, Galloway TS. Dynamics of nuclear receptor gene expression during Pacific oyster development. BMC Dev Biol BMC Develop Biol. 2016;16:1–13.

    CAS  Google Scholar 

  22. Li Z, Nicolae V, Akileh R, Liu T. A brief review of oyster-associated microbiota. Microbiol Res J Int. 2017;20:1–14.

    Article  CAS  Google Scholar 

  23. Stevick RJ, Sohn S, Modak TH, Nelson DR, Rowley DC, Tammi K, et al. Bacterial community dynamics in an oyster hatchery in response to probiotic treatment. Front Microbiol. 2019;10:1–13.

    Article  Google Scholar 

  24. King GM, Judd C, Kuske CR, Smith C. Analysis of stomach and gut microbiomes of the eastern oyster (Crassostrea virginica) from Coastal Louisiana, USA. PLoS ONE. 2012;7: e51475.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Lokmer A, Mathias WK. Hemolymph microbiome of Pacific oysters in response to temperature, temperature stress and infection. ISME J. 2015;9:670–82.

    Article  CAS  PubMed  Google Scholar 

  26. Wegner KM, Volkenborn N, Peter H, Eiler A. Disturbance induced decoupling between host genetics and composition of the associated microbiome. BMC Microbiol. 2013;13:252.

    Article  PubMed  PubMed Central  Google Scholar 

  27. Fisheries and Agriculture Organisation of the UN (FAO). The state of world fisheries and aquaculture 2018. Meeting the sustainable development goals. 2018: Rome, Italy. Rome; 2018.

  28. Brusca RC, Brusca GJ. Invertebrates. 2nd ed. Massachussetts: Sinauer Associates; 2003.

    Google Scholar 

  29. Jefferson R. Aquauclture production report 2017–2018. NSW, Australia; 2019.

  30. Gippel E. Aquaculture production report. NSW, Australia; 2021.

  31. Scanes E, Parker LM, Seymour JR, Siboni N, King WL, Wegner KM, et al. Microbiome response differs among selected lines of Sydney rock oysters to ocean warming and acidification. Oxford University Press; 2021. p. 1–13.

    Google Scholar 

  32. Parker LM, O’Connor WA, Byrne M, Coleman RA, Virtue P, Dove M, et al. Adult exposure to ocean acidification is maladaptive for larvae of the Sydney rock oyster Saccostrea glomerata in the presence of multiple stressors. Biol Lett. 2017;13:9–13.

    Article  Google Scholar 

  33. O’Connor WA, Dove MC, Finn B, O’Connor S. Manual for hatchery production of Sydney rock oyster (Saccostrea glomerata). Final report to Fisheries Research and Development Corporation, Deakin, ACT, Australia. New South Wales Department of Primary Industries - Fisheries Research Report Series. 2008:20. p. 55.

  34. Parada AE, Needham DM, Fuhrman JA. Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. Environ Microbiol. 2016;18:1403–14.

    Article  CAS  PubMed  Google Scholar 

  35. Apprill A, Mcnally S, Parsons R, Weber L. Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton. Aquat Microb Ecol. 2015;75:129–37.

    Article  Google Scholar 

  36. Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJA, Holmes SP. DADA2: high-resolution sample inference from Illumina amplicon data. Nat Methods. 2016;13:581–3.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, et al. The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools. Nucleic Acids Res. 2012;41:590–6.

    Article  CAS  Google Scholar 

  38. McMurdie PJ, Holmes S. Phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data. PLoS ONE. 2013;8:e61217.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Davis N, Proctor D, Holmes S, Relman D, Callahan B. Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data. Simple Stat Identif Remov Contam Seq marker-gene metagenomics data. Microbiome; 2018;221499.

  40. Karstens L, Asquith M, Davin S, Fair D, Gregory WT, Wolfe AJ, et al. Sequencing experiments. mSystems. 2019;4:1–14.

    Google Scholar 

  41. Bullard JH, Purdom E, Hansen KD, Dudoit S. Evaluation of statistical methods for normalization and differential expression in mRNA-Seq experiments. BMC Bioinformatics. 2010;11.

  42. Dillies MA, Rau A, Aubert J, Hennequet-Antier C, Jeanmougin M, Servant N, et al. A comprehensive evaluation of normalization methods for Illumina high-throughput RNA sequencing data analysis. Brief Bioinform. 2013;14:671–83.

    Article  CAS  PubMed  Google Scholar 

  43. Weiss S, Xu ZZ, Peddada S, Amir A, Bittinger K, Gonzalez A, et al. Normalization and microbial differential abundance strategies depend upon data characteristics. Microbiome Microbiome. 2017;5:1–18.

    Google Scholar 

  44. McMurdie PJ, Holmes S. Waste not, want not: why rarefying microbiome data is inadmissible. PLoS Comput Biol. 2014;10.

  45. McKnight DT, Huerlimann R, Bower DS, Schwarzkopf L, Alford RA, Zenger KR. Methods for normalizing microbiome data: an ecological perspective. Methods Ecol Evol. 2019;10:389–400.

    Article  Google Scholar 

  46. Oksanen J, Blanchet F, Friendly M, Knidt R, Legendre P, Minchin P. vegan: Community ecology package. R package version 2.5-7;2020.

  47. Martinez A. pairwiseAdonis: pairwise comparison using Adonis. R package version 0.4. 2020.

  48. Buttigieg PL, Ramette A. A guide to statistical analysis in microbial ecology: a community-focused, living review of multivariate data analyses. FEMS Microbiol Ecol. 2014;90:543–50.

    Article  CAS  PubMed  Google Scholar 

  49. Bastian M, Heymann S, Jacomy M. Gephi: an open source software for exploring and manipulating networks. International AAAI conference on weblogs and social media;2009.

  50. Liaw A, Wiener M. Classification and regression by randomForest. R News;2002.

  51. Kuhn M. A Short Introduction to the caret Package. R Found Stat Comput. 2016;1–10.

  52. Miller CA, Holm HC, Horstmann L, George JC, Fredricks HF, Van Mooy BAS, et al. Coordinated transformation of the gut microbiome and lipidome of bowhead whales provides novel insights into digestion. ISME J. 2020;14:688–701.

    Article  CAS  PubMed  Google Scholar 

  53. Lahti L, Shetty S. microbiome R package. 2017.

  54. Wickham H. ggplot2: elegant graphics for data analysis. New York: Springer; 2016.

    Book  Google Scholar 

  55. Xiao F, Zhu W, Yu Y, He Z, Wu B, Wang C, et al. Host development overwhelms environmental dispersal in governing the ecological succession of zebrafish gut microbiota. npj Biofilms Microbiomes. 2021;7.

  56. Stephens WZ, Burns AR, Stagaman K, Wong S, Rawls JF, Guillemin K, et al. The composition of the zebrafish intestinal microbial community varies across development. ISME J. 2016;10:644–54.

    Article  PubMed  CAS  Google Scholar 

  57. Wang Y, Wang K, Huang L, Dong P, Wang S, Chen H, et al. Fine-scale succession patterns and assembly mechanisms of bacterial community of Litopenaeus vannamei larvae across the developmental cycle. Microbiome Microbiome. 2020;8:1–16.

    Google Scholar 

  58. Arfken A, Song B, Allen SK, Carnegie RB. Comparing larval microbiomes of the eastern oyster (Crassostrea virginica) raised in different hatcheries. Aquaculture. 2021;531: 735955.

    Article  CAS  Google Scholar 

  59. Carrier TJ, Reitzel AM. Bacterial community dynamics during embryonic and larval development of three confamilial echinoids. Mar Ecol Prog Ser. 2019;611:179–88.

    Article  CAS  Google Scholar 

  60. Fieth RA, Gauthier MEA, Bayes J, Green KM, Degnan SM. Ontogenetic changes in the bacterial symbiont community of the tropical demosponge Amphimedon queenslandica: metamorphosis is a new beginning. Front Mar Sci. 2016;3:1–20.

    Article  Google Scholar 

  61. Laroche O, Symonds JE, Smith KF, Banks JC, Mae H, Bowman JP, et al. Understanding bacterial communities for informed biosecurity and improved larval survival in Pacific oysters. Aquaculture. 2018;497:164–73.

    Article  Google Scholar 

  62. Gil-turnes MS, Hay ME, Fenical W. Symbiotic marine bacteria chemically defend crustacean embryos from a pathogenic fungus. Science. 1989;246:116–8.

    Article  CAS  PubMed  Google Scholar 

  63. Lema KA, Willis BL, Bourneb DG. Corals form characteristic associations with symbiotic nitrogen-fixing bacteria. Appl Environ Microbiol. 2012;78:3136–44.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  64. Pujalte MJ, Lucena T, Ruvira M, Arahal DR, Macian MC. The family rhodobacteraceae. The prokaryotes. Berlin: Springer; 2014. p. 439–512.

    Book  Google Scholar 

  65. Dogs M, Wemheuer B, Wolter L, Bergen N, Daniel R, Simon M, et al. Rhodobacteraceae on the marine brown alga Fucus spiralis are abundant and show physiological adaptation to an epiphytic lifestyle. Syst Appl Microbiol. 2017;40:370–82.

    Article  CAS  PubMed  Google Scholar 

  66. MacNeil LT, Watson E, Arda HE, Zhu LJ, Walhout AJM. Diet-induced developmental acceleration independent of TOR and insulin in C. elegans. Cell. 2013;153:240–52.

    Article  CAS  PubMed  Google Scholar 

  67. Watson E, Macneil LT, Ritter AD, Yilmaz LS, Rosebrock AP, Caudy AA, et al. Interspecies systems biology uncovers metabolites affecting C. elegans gene expression and life history traits. Cell. 2014;156:759–70.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  68. Sacristán-Soriano O, Winkler M, Erwin P, Weisz J, Harriott O, Heussler G, et al. Ontogeny of symbiont community structure in two carotenoid-rich, viviparous marine sponges: comparison of microbiomes and analysis of culturable pigmented heterotrophic bacteria. Environ Microbiol Rep. 2019;11:249–61.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  69. Asmani K, Petton B, Le Grand J, Mounier J, Robert R, Nicolas JL. Establishment of microbiota in larval culture of Pacific oyster. Crassostrea gigas Aquacult. 2016;464:434–44.

    Article  Google Scholar 

  70. Wilkes Walburn J, Wemheuer B, Thomas T, Copeland E, O’Connor W, Booth M, et al. Diet and diet-associated bacteria shape early microbiome development in Yellowtail Kingfish (Seriola lalandi). Microb Biotechnol. 2019;12:275–88.

    Article  CAS  PubMed  Google Scholar 

  71. Hammer TJ, Janzen DH, Hallwachs W, Jaffe SP, Fierer N. Caterpillars lack a resident gut microbiome. PNAS. 2017;114:9641–6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  72. Collins AJ, LaBarre BA, Won BSW, Shah MV, Heng S, Choudhury MH, et al. Diversity and partitioning of bacterial populations within the accessory nidamental gland of the squid Euprymna scolopes. Appl Environ Microbiol. 2012;78:4200–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  73. Kerwin AH, Nyholm SV. Symbiotic bacteria associated with a bobtail squid reproductive system are detectable in the environment, and stable in the host and developing eggs. Environ Microbiol. 2017;19:1463–75.

    Article  PubMed  Google Scholar 

  74. Desriac F, El Harras A, Simon M, Bondon A, Brillet B, Le Chevalier P, et al. Alterins produced by oyster-associated pseudoalteromonas are antibacterial cyclolipopeptides with LPS-binding activity. Mar Drugs. 2020;18.

  75. Monga M, Roberts JA. Spermagglutination by bacteria: receptor-specific interactions. J Androl. 1994;15:151–6.

    CAS  PubMed  Google Scholar 

  76. Kalinovskaya NI, Ivanova EP, Alexeeva YV, Gorshkova NM, Kuznetsova TA, Dmitrenok AS, et al. Low-molecular-weight, biologically active compounds from marine Pseudoalteromonas species. Curr Microbiol. 2004;48:441–6.

    Article  CAS  PubMed  Google Scholar 

  77. Boulais M, Suquet M, Arsenault-Pernet EJ, Malo F, Queau I, Pignet P, et al. PH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas). Biol Open. 2018;7:3–9.

    Google Scholar 

  78. Baud D, Pattaroni C, Vulliemoz N, Castella V, Marsland BJ, Stojanov M. Sperm microbiota and its impact on semen parameters. Front Microbiol. 2019;10:1–9.

    Article  Google Scholar 

  79. Marco-Jiménez F, Borrás S, Garcia-Dominguez X, D’Auria G, Vicente JS, Marin C. Roles of host genetics and sperm microbiota in reproductive success in healthy rabbit. Theriogenology. 2020;158:416–23.

    Article  PubMed  CAS  Google Scholar 

  80. Jensen S, Duperron S, Birkeland NK, Hovland M. Intracellular Oceanospirillales bacteria inhabit gills of Acesta bivalves. FEMS Microbiol Ecol. 2010;74:523–33.

    Article  CAS  PubMed  Google Scholar 

  81. Zurel D, Benayahu Y, Or A, Kovacs A, Gophna U. Composition and dynamics of the gill microbiota of an invasive Indo-Pacific oyster in the eastern Mediterranean Sea. Environ Microbiol. 2011;13:1467–76.

    Article  PubMed  Google Scholar 

  82. Beinart RA, Nyholm SV, Dubilier N, Girguis PR. Intracellular Oceanospirillales inhabit the gills of the hydrothermal vent snail Alviniconcha with chemosynthetic, γ-Proteobacterial symbionts. Environ Microbiol Rep. 2014;6:656–64.

    Article  CAS  PubMed  Google Scholar 

  83. Hanzen TC, Dubisky EA, DeSantis TZ, Andersen GL, Piceno YM, Singh N, et al. Deep-sea oil plume enriches indigenous oil-degrading bacteria. Science. 2010;330:204–9.

    Article  CAS  Google Scholar 

  84. Dubinsky EA, Conrad ME, Chakraborty R, Bill M, Borglin SE, Hollibaugh JT, et al. Succession of hydrocarbon-degrading bacteria in the aftermath of the deepwater horizon oil spill in the Gulf of Mexico. Environ Sci Technol. 2013;47:10860–7.

    Article  CAS  PubMed  Google Scholar 

  85. Pinhassi J, Pujalte MJ, Pascual J, González JM, Lekunberri I, Pedrós-Alió C, et al. Bermanella marisrubri gen. nov., sp. Nov., a genome-sequenced gammaproteobacterium from the Red Sea. Int J Syst Evol Microbiol. 2009;59:373–7.

    Article  CAS  PubMed  Google Scholar 

  86. Suriyamongkol P, Weselake R, Narine S, Moloney M, Shah S. Biotechnological approaches for the production of polyhydroxyalkanoates in microorganisms and plants—a review. Biotechnol Adv. 2007;25:148–75.

    Article  CAS  PubMed  Google Scholar 

  87. Wang RY, Shi ZY, Chen JC, Wu Q, Chen GQ. Enhanced co-production of hydrogen and poly-(R)-3-hydroxybutyrate by recombinant PHB producing E. coli over-expressing hydrogenase 3 and acetyl-CoA synthetase. Metab Eng. 2012;14:496–503.

    Article  CAS  PubMed  Google Scholar 

  88. De Schryver P, Sinha AK, Kunwar PS, Baruah K, Verstraete W, Boon N, et al. Poly-β-hydroxybutyrate (PHB) increases growth performance and intestinal bacterial range-weighted richness in juvenile European sea bass. Dicentrarchus labrax Appl Microbiol Biotechnol. 2010;86:1535–41.

    Article  CAS  PubMed  Google Scholar 

  89. Laranja JLQ, Ludevese-Pascual GL, Amar EC, Sorgeloos P, Bossier P, De Schryver P. Poly-β-hydroxybutyrate (PHB) accumulating Bacillus spp. improve the survival, growth and robustness of Penaeus monodon (Fabricius, 1798) postlarvae. Vet Microbiol. 2014;173:310–7.

    Article  CAS  PubMed  Google Scholar 

  90. Najdegerami EH, Baruah K, Shiri A, Rekecki A, Van den Broeck W, Sorgeloos P, et al. Siberian sturgeon (Acipenser baerii) larvae fed Artemia nauplii enriched with poly-β-hydroxybutyrate (PHB): effect on growth performance, body composition, digestive enzymes, gut microbial community, gut histology and stress tests. Aquac Res. 2015;46:801–12.

    Article  CAS  Google Scholar 

  91. Sui L, Cai J, Sun H, Wille M, Bossier P. Effect of poly-β-hydroxybutyrate on Chinese mitten crab, Eriocheir sinensis, larvae challenged with pathogenic Vibrio anguillarum. J Fish Dis. 2012;35:359–64.

    Article  CAS  PubMed  Google Scholar 

  92. Baruah K, Huy TT, Norouzitallab P, Niu Y, Gupta SK, De Schryver P, et al. Probing the protective mechanism of poly-β-hydroxybutyrate against vibriosis by using gnotobiotic Artemia franciscana and Vibrio campbellii as host-pathogen model. Sci Rep. 2015;5:1–8.

    Article  Google Scholar 

  93. Funkhouser LJ, Bordenstein SR. Mom knows best: the universality of maternal microbial transmission. PLoS Biol. 2013;11:1–9.

    Article  CAS  Google Scholar 

  94. Paz LC, Schramm A, Lund MB. Biparental transmission of Verminephrobacter symbionts in the earthworm Aporrectodea tuberculata (Lumbricidae). FEMS Microbiol Ecol. 2017;93:1–8.

    Article  CAS  Google Scholar 

  95. Cross ST, Maertens BL, Dunham TJ, Rodgers CP, Brehm AL, Miller MR, et al. Partitiviruses infecting Drosophila melanogaster and Aedes aegypti exhibit efficient biparental vertical transmission. J Virol. 2020;94:e01070-e1120.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  96. Björk JR, Díez-vives C, Astudillo-garcía C, Archie EA, Montoya JM. Vertical transmission of sponge micrbiota is inconsistent and unfaithful. Nat Ecol Evol. 2019;3:1172–83.

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The authors would like to thank the staff at Port Stephens Fisheries Institute, including, Stephan O’Connor, Greg Kent, Kyle Johnston, and Brandt Archer, for their help with the experiment and algal culture.

Funding

This manuscript is based on work supported by the National Science Foundation Graduate Research Opportunities Worldwide (GROW) Grant no. 1938052 and National Science Foundation Graduate Research Fellowship Program (GRFP) under Grant no. 1451070.

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AU-M, JLB, PMR, and WO conceptualized, designed, and procured funding for the study. AU-M, under the guidance of JLB, conducted the experiment, collected and processed samples, conducted statistical analyses and led the writing of the manuscript. ES and LMP conducted the experiment and collected samples. All authors contributed to the writing of and approved the manuscript. All authors read and approved the final manuscript.

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Correspondence to Andrea Unzueta-Martínez.

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Unzueta-Martínez, A., Scanes, E., Parker, L.M. et al. Microbiomes of the Sydney Rock Oyster are acquired through both vertical and horizontal transmission. anim microbiome 4, 32 (2022). https://doi.org/10.1186/s42523-022-00186-9

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