Fig. 2

Experimental microbial environmental effects on egg immune function. A Lysozyme concentration (mg ml⁻¹; log scale), B Ovotransferrin concentration (mg  ml⁻¹), C IgY concentration in yolk (OD_405nm), and D the first two principal coordinate axes of a multivariate immune index that represents the variation of the indices presented in A–C. Individual egg samples are presented by laying sequence (color) and stratified by clutch number (shape in A–C) or treatment (shape in D). None of the egg immune indices were significantly different between the two experimental microbial environments (Table 1)